Circumoval and Circumlarval Precipitate Reactions of Angiostrongylus Cantoneaisjs
نویسنده
چکیده
Using sera obtained from Angi'ostroirglylus cantonensis-infected or -transferred rats and immunized guinea pigs with A. cantonensis hemogenate, positive circumoval and circumlarval precipitate reactions on living A. cantonensis at various developmental stages were recognized after incubation at 340C for 24hr. Characteristic precipitates were mainly formed on the excretory pore and cuticle of the 3rd-stage larvae; the oral opening, excretory pore and cuticle of the 4th-stage larvae ; and the vulva, anus, cloacal and oral openings, excretory pore and cuticle of immature adults. However, precipitates were not formed on the living lst-stage !arvae. Precipitates began to appear 1 to 4 weeks after infection, These reactions were recognized irrespective of the sex and developrnental stage of the worm. Pesitive cross-reactions were observed in the sera from A. costaricensis-infected rats. Applying a technique of immunofluorescence, it was
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